Texas A&M College of Agriculture and Life Sciences
Pellois Lab

Publications

  1. Park, G.; Brock, D. J.; Pellois, J.-P.; Gabbaï, F. P. Heavy Pnictogenium Cations as Transmembrane Anion Transporters in Vesicles and Erythrocytes. Chem 2019, 5 (8), 2215–2227. https://doi.org/10.1016/J.CHEMPR.2019.06.013.
  2. Pellois, J.-P. Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery
    Tools Can Contribute to Precise and Quantitative Cell Biology Assays. BioEssays 2019, 41 (6). https://doi.org/10.1002/bies.201900031.
  3. Brock, D. J.; Kondow-Mcconaghy, H. M.; Hager, E. C.; Pellois, J.-P. Endosomal Escape and Cytosolic Penetration of Macromolecules Mediated by Synthetic Delivery Agents. Bioconjug. Chem. 2019, 30 (2), 293–304. https://doi.org/10.1021/acs.bioconjchem.8b00799.
  4. Fang, Y.; Lian, X.; Huang, Y.; Fu, G.; Xiao, Z.; Wang, Q.; Nan, B.; Pellois, J.-P.; Zhou, H.-C. Investigating Subcellular Compartment Targeting Effect of Porous Coordination Cages for Enhancing Cancer Nanotherapy. Small 2018, 14 (47). https://doi.org/10.1002/smll.201802709.
  5. Allen, J. K.; Brock, D. J.; Kondow-McConaghy, H. M.; Pellois, J.-P. Efficient Delivery of Macromolecules into Human Cells by Improving the Endosomal Escape Activity of Cell-Penetrating Peptides: Lessons Learned from DfTAT and Its Analogs. Biomolecules 2018, 8 (3). https://doi.org/10.3390/biom8030050.
  6. Brock, D. J.; Kustigian, L.; Jiang, M.; Graham, K.; Wang, T.-Y.; Erazo-Oliveras, A.; Najjar, K.; Zhang, J.; Rye, H.; Pellois, J.-P. Efficient Cell Delivery Mediated by Lipid-Specific Endosomal Escape of Supercharged Branched Peptides. Traffic 2018, 19 (6), 421–435. https://doi.org/10.1111/tra.12566.
  7. Lian, X.; Huang, Y.; Zhu, Y.; Fang, Y.; Zhao, R.; Joseph, E.; Li, J.; Pellois, J.-P.; Zhou, H.-C. Enzyme-MOF Nanoreactor Activates Nontoxic Paracetamol for Cancer Therapy. Angew. Chemie – Int. Ed. 2018, 57 (20), 5725–5730. https://doi.org/10.1002/anie.201801378.
  8. Stubbendieck, R. M.; Brock, D. J.; Pellois, J.-P.; Gill, J. J.; Straight, P. D. Linearmycins Are Lytic Membrane-Targeting Antibiotics. J. Antibiot. (Tokyo). 2018, 71 (3), 372–381. https://doi.org/10.1038/s41429-017-0005-z.
  9. Lian, X.; Erazo-Oliveras, A.; Pellois, J.-P.; Zhou, H.-C. High Efficiency and Long-Term Intracellular Activity of an Enzymatic Nanofactory Based on Metal-Organic Frameworks. Nat. Commun. 2017, 8 (1). https://doi.org/10.1038/s41467-017-02103-0.
  10. Najjar, K.; Erazo-Oliveras, A.; Mosior, J. W.; Whitlock, M. J.; Rostane, I.; Cinclair, J. M.; Pellois, J.-P. Unlocking Endosomal Entrapment with Supercharged Arginine-Rich Peptides. Bioconjug. Chem. 2017, 28 (12), 2932–2941. https://doi.org/10.1021/acs.bioconjchem.7b00560.
  11. Libardo, M. D. J.; Wang, T.-Y.; Pellois, J.-P.; Angeles-Boza, A. M. How Does Membrane Oxidation Affect Cell Delivery and Cell Killing? Trends Biotechnol. 2017, 35 (8), 686–690. https://doi.org/10.1016/j.tibtech.2017.03.015.
  12. Wang, T.-Y.; Libardo, M. D. J.; Angeles-Boza, A. M.; Pellois, J.-P. Membrane Oxidation in Cell Delivery and Cell Killing Applications. ACS Chem. Biol. 2017, 12 (5), 1170–1182. https://doi.org/10.1021/acschembio.7b00237.
  13. Najjar, K.; Erazo-Oliveras, A.; Brock, D. J.; Wang, T.-Y.; Pellois, J.-P. An L- to D-Amino Acid Conversion in an Endosomolytic Analog of the Cell-Penetrating Peptide TAT Influences Proteolytic Stability, Endocytic Uptake, and Endosomal Escape. J. Biol. Chem. 2017, 292 (3), 847–861. https://doi.org/10.1074/jbc.M116.759837.
  14. Robison, A. D.; Sun, S.; Poyton, M. F.; Johnson, G. A.; Pellois, J.-P.; Jungwirth, P.; Vazdar, M.; Cremer, P. S. Polyarginine Interacts More Strongly and Cooperatively than Polylysine with Phospholipid Bilayers. J. Phys. Chem. B 2016, 120 (35), 9287–9296. https://doi.org/10.1021/acs.jpcb.6b05604.
  15. Erazo-Oliveras, A.; Najjar, K.; Truong, D.; Wang, T.-Y.; Brock, D. J.; Prater, A. R.; Pellois, J.-P. The Late Endosome and Its Lipid BMP Act as Gateways for Efficient Cytosolic Access of the Delivery Agent DfTAT and Its Macromolecular Cargos. Cell Chem. Biol. 2016, 23 (5), 598–607. https://doi.org/10.1016/j.chembiol.2016.03.016.
  16. Wang, T.-Y.; Sun, Y.; Muthukrishnan, N.; Erazo-Oliveras, A.; Najjar, K.; Pellois, J.-P. Membrane Oxidation Enables the Cytosolic Entry of Polyarginine Cell-Penetrating Peptides. J. Biol. Chem. 2016, 291 (15), 7902–7914. https://doi.org/10.1074/jbc.M115.711564.
  17. Najjar, K.; Erazo-Oliveras, A.; Pellois, J.-P. Delivery of Proteins, Peptides or Cell-Impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent DfTAT. J. Vis. Exp. 2015, 2015 (103). https://doi.org/10.3791/53175.
  18. Patterson, J. L.; Arenas-Gamboa, A. M.; Wang, T.-Y.; Hsiao, H.-C.; Howell, D. W.; Pellois, J.-P.; Rice-Ficht, A.; Bondos, S. E. Materials Composed of the Drosophila Hox Protein Ultrabithorax Are Biocompatible and Nonimmunogenic. J. Biomed. Mater. Res. – Part A 2015, 103 (4), 1546–1553. https://doi.org/10.1002/jbm.a.35295.
  19. Li, Z.; David, A.; Albani, B. A.; Pellois, J.-P.; Turro, C.; Dunbar, K. R. Optimizing the Electronic Properties of Photoactive Anticancer Oxypyridine-Bridged Dirhodium(II,II) Complexes. J. Am. Chem. Soc. 2014, 136 (49), 17058–17070. https://doi.org/10.1021/ja5078359.
  20. Peña, B.; David, A.; Pavani, C.; Baptista, M. S.; Pellois, J.-P.; Turro, C.; Dunbar, K. R. Cytotoxicity Studies of Cyclometallated Ruthenium(II) Compounds: New Applications for Ruthenium Dyes. Organometallics 2014, 33 (5), 1100–1103. https://doi.org/10.1021/om500001h.
  21. Johnson, G. A.; Ellis, E. A.; Kim, H.; Muthukrishnan, N.; Snavely, T.; Pellois, J.-P. Photoinduced Membrane Damage of E. Coli and S. Aureus by the Photosensitizer-Antimicrobial Peptide Conjugate Eosin-(KLAKLAK)2. PLoS One 2014, 9 (3). https://doi.org/10.1371/journal.pone.0091220.
  22. Muthukrishnan, N.; Donovan, S.; Pellois, J.-P. The Photolytic Activity of Poly-Arginine Cell Penetrating Peptides Conjugated to Carboxy-Tetramethylrhodamine Is Modulated by Arginine Residue Content and Fluorophore Conjugation Site. Photochem. Photobiol. 2014, 90 (5), 1034–1042. https://doi.org/10.1111/php.12288.
  23. Meerovich, I.; Muthukrishnan, N.; Johnson, G. A.; Erazo-Oliveras, A.; Pellois, J.-P. Photodamage of Lipid Bilayers by Irradiation of a Fluorescently Labeled Cell-Penetrating Peptide. Biochim. Biophys. Acta – Gen. Subj. 2014, 1840 (1), 507–515. https://doi.org/10.1016/j.bbagen.2013.10.011.
  24. Erazo-Oliveras, A.; Najjar, K.; Dayani, L.; Wang, T.-Y.; Johnson, G. A.; Pellois, J.-P. Protein Delivery into Live Cells by Incubation with an Endosomolytic Agent. Nat. Methods 2014, 11 (8), 861–867. https://doi.org/10.1038/nMeth.2998.
  25. Muthukrishnan, N.; Johnson, G. A.; Erazo-Oliveras, A.; Pellois, J.-P. Synergy between Cell-Penetrating Peptides and Singlet Oxygen Generators Leads to Efficient Photolysis of Membranes. Photochem. Photobiol. 2013, 89 (3), 625–630. https://doi.org/10.1111/php.12036.
  26. Johnson, G. A.; Muthukrishnan, N.; Pellois, J.-P. Photoinactivation of Gram Positive and Gram Negative Bacteria with the Antimicrobial Peptide (KLAKLAK)2 Conjugated to the Hydrophilic Photosensitizer Eosin Y. Bioconjug. Chem. 2013, 24 (1), 114–123. https://doi.org/10.1021/bc3005254.
  27. Muthukrishnan, N.; Johnson, G. A.; Lim, J.; Simanek, E. E.; Pellois, J.-P. TAT-Mediated Photochemical Internalization Results in Cell Killing by Causing the Release of Calcium into the Cytosol of Cells. Biochim. Biophys. Acta – Gen. Subj. 2012, 1820 (11), 1734–1743. https://doi.org/10.1016/j.bbagen.2012.06.020.
  28. Erazo-Oliveras, A.; Muthukrishnan, N.; Baker, R.; Wang, T.-Y.; Pellois, J.-P. Improving the Endosomal Escape of Cell-Penetrating Peptides and Their Cargos: Strategies and Challenges. Pharmaceuticals 2012, 5 (11), 1177–1209. https://doi.org/10.3390/ph5111177.
  29. Lee, Y.-J.; Johnson, G.; Peltier, G. C.; Pellois, J.-P. A HA2-Fusion Tag Limits the Endosomal Release of Its Protein Cargo despite Causing Endosomal Lysis. Biochim. Biophys. Acta – Gen. Subj. 2011, 1810 (8), 752–758. https://doi.org/10.1016/j.bbagen.2011.05.013.
  30. Srinivasan, D.; Muthukrishnan, N.; Johnson, G. A.; Erazo-Oliveras, A.; Lim, J.; Simanek, E. E.; Pellois, J.-P. Conjugation to the Cell-Penetrating Peptide TAT Potentiates the Photodynamic Effect of Carboxytetramethylrhodamine. PLoS One 2011, 6 (3). https://doi.org/10.1371/journal.pone.0017732.
  31. Angeles-Boza, A. M.; Erazo-Oliveras, A.; Lee, Y.-J.; Pellois, J.-P. Generation of Endosomolytic Reagents by Branching of Cell-Penetrating Peptides: Tools for the Delivery of Bioactive Compounds to Live Cells in Cis or Trans. Bioconjug. Chem. 2010, 21 (12), 2164–2167. https://doi.org/10.1021/bc100130r.
  32. Lim, J.; Pellois, J.-P.; Simanek, E. E. A Retro-Inverso TAT-like Peptide Designed to Deliver Cysteamine to Cells. Bioorganic Med. Chem. Lett. 2010, 20 (21), 6321–6323. https://doi.org/10.1016/j.bmcl.2010.07.046.
  33. Lee, Y.-J.; Johnson, G.; Pellois, J.-P. Modeling of the Endosomolytic Activity of HA2-TAT Peptides with Red Blood Cells and Ghosts. Biochemistry 2010, 49 (36), 7854–7866. https://doi.org/10.1021/bi1008408.
  34. Lee, Y.-J.; Erazo-Oliveras, A.; Pellois, J.-P. Delivery of Macromolecules into Live Cells by Simple Co-Incubation with a Peptide. ChemBioChem 2010, 11 (3), 325–330. https://doi.org/10.1002/cbic.200900527.
  35. Aguirre, J. D.; Angeles-Boza, A. M.; Chouai, A.; Turro, C.; Pellois, J.-P.; Dunbar, K. R. Anticancer Activity of Heteroleptic Diimine Complexes of Dirhodium: A Study of Intercalating Properties, Hydrophobicity and in Cellulo Activity. Dalt. Trans. 2009, No. 48, 10806–10812. https://doi.org/10.1039/b915357h.
  36. Aguirre, J. D.; Angeles-Boza, A. M.; Chouai, A.; Pellois, J.-P.; Turro, C.; Dunbar, K. R. Live Cell Cytotoxicity Studies: Documentation of the Interactions of Antitumor Active Dirhodium Compounds with Nuclear DNA. J. Am. Chem. Soc. 2009, 131 (32), 11353–11360. https://doi.org/10.1021/ja9021717.
  37. Loudet, A.; Han, J.; Barhoumi, R.; Pellois, J.-P.; Burghardt, R. C.; Burgess, K. Non-Covalent Delivery of Proteins into Mammalian Cells. Org. Biomol. Chem. 2008, 6 (24), 4516–4522. https://doi.org/10.1039/b809006h.
  38. Lee, Y.-J.; Datta, S.; Pellois, J.-P. Real-Time Fluorescence Detection of Protein Transduction into Live Cells. J. Am. Chem. Soc. 2008, 130 (8), 2398–2399. https://doi.org/10.1021/ja7102026.
  39. Hahn, M. E.; Pellois, J.-P.; Vila-Perelló, M.; Muir, T. W. Tunable Photoactivation of a Post-Translationally Modified Signaling Protein and Its Unmodified Counterpart in Live Cells. ChemBioChem 2007, 8 (17), 2100–2105. https://doi.org/10.1002/cbic.200700404.
  40. Pellois, J.-P.; Muir, T. W. Semisynthetic Proteins in Mechanistic Studies: Using Chemistry to Go Where Nature Can’t. Curr. Opin. Chem. Biol. 2006, 10 (5), 487–491. https://doi.org/10.1016/j.cbpa.2006.08.016.
  41. Pellois, J.-P.; Muir, T. W. A Ligation and Photorelease Strategy for the Temporal and Spatial Control of Protein Function in Living Cells. Angew. Chemie – Int. Ed. 2005, 44 (35), 5713–5717. https://doi.org/10.1002/anie.200501244.
  42. Gao, X.; Pellois, J. P.; Na, Y.; Kim, Y.; Gulari, E.; Zhou, X. High Density Peptide Microarrays. In Situ Synthesis and Applications. Mol. Divers. 2004, 8 (3), 177–187. https://doi.org/10.1023/B:MODI.0000036233.58271.25.
  43. Pellois, J.-P.; Hahn, M. E.; Muir, T. W. Simultaneous Triggering of Protein Activity and Fluorescence. J. Am. Chem. Soc. 2004, 126 (23), 7170–7171. https://doi.org/10.1021/ja0499142.
  44. Srivannavit, O.; Gulari, M.; Gulari, E.; LeProust, E.; Pellois, J. P.; Gao, X.; Zhou, X. Design and Fabrication of Microwell Array Chips for a Solution-Based, Photogenerated Acid-Catalyzed Parallel Oligonuclotide DNA Synthesis. Sensors Actuators, A Phys. 2004, 116 (1), 150–160. https://doi.org/10.1016/j.sna.2004.04.025.
  45. Pellois, J. P.; Zhou, X.; Srivannavit, O.; Zhou, T.; Gulari, E.; Gao, X. Individually Addressable Parallel Peptide Synthesis on Microchips. Nat. Biotechnol. 2002, 20 (9), 922–926. https://doi.org/10.1038/nbt723.
  46. LeProust, E.; Pellois, J. P.; Yu, P.; Zhang, H.; Gao, X.; Srivannavit, O.; Gulari, E.; Zhou, X. Digital Light-Directed Synthesis. A Microarray Platform That Permits Rapid Reaction Optimization on a Combinatorial Basis. J. Comb. Chem. 2000, 2 (4), 349–354. https://doi.org/10.1021/cc000009x.
  47. Pellois, J. P.; Wang, W.; Gao, X. Peptide Synthesis Based on T-Boc Chemistry and Solution Photogenerated Acids. J. Comb. Chem. 2000, 2 (4), 355–360.
  48. Gao, X.; Yu, P.; LeProust, E.; Sonigo, L.; Pellois, J. P.; Zhang, H. Oligonucleotide Synthesis Using Solution Photogenerated Acids [17]. J. Am. Chem. Soc. 1998, 120 (48), 12698–12699. https://doi.org/10.1021/ja9827191.